RK13細(xì)胞

ATCC Number： CCL-37?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 正常

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 其他

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來(lái)源： 腎臟

數(shù)量： 大量

細(xì)胞形態(tài)： 上皮樣

年限： 5 weeks

RK13細(xì)胞Designations: RK13

Depositors: BC Meyer

Biosafety Level: 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Oryctolagus cuniculus

Morphology: epithelial

Source: Organ: kidney

Disease: normal

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RK13細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Age: 5 weeks

Comments: The cells are positive for keratin by immunoperoxidase staining.The cells are positive for bovine viral diarrhea virus (BVDV).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: RK13細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. RK13細(xì)胞J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

22145: . . Lancet 2: 640, 1963.

22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

26192: . . Lancet 2: 593, 1963.

28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321