NFS-5 C-1細(xì)胞
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
品系： NFS/N
ATCC Number： CRL-1693?
相關(guān)**： **瘤
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: NFS-5 C-1
Depositors: WJ Davidson, HC Morse
NFS-5 C-1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: NFS/N
Cell Type: pre-B lymphoblast;
Cellular Products: ecotropic retrovirus; mink cell focus forming retrovirus
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.NFS-5 C-1細(xì)胞 Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD5 +; CD32 +; CD45R +
Comments: The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.
The cells bear a pattern of markers consistent with identification as large pre-B lymphoblasts (Ly 17, CD32.
Ly 1, CD5.
Lyb 2, Ly 5(B220), CD45R).
They are negative for ThB, surface immunoglobulin (sIg) and Ia.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Modified Dulbecco's medium with 0.002 mg/ml transferrin and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
NFS-5 C-1細(xì)胞Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22836: Davidson WF, et al. NFS-5 C-1細(xì)胞A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201
