5H8細(xì)胞

數(shù)量： 大量

ATCC Number： CRL-2646?

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG1; kappa light chain

Designations: 5H8

5H8細(xì)胞Depositors: LB Chen

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against periostin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 5H8細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The antibody reacts with periostin, a secreted protein. The levels of serum periostin may be an indicator of tumor invasion and progression in patients with thymoma and nonsmall cell lung carcinomas (NSCLC).

Animals were immunized with Fc-TCG1, which is similar to periostsin.

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Growth Conditions: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

Medium Renewal: 5H8細(xì)胞Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: A 1:1 mixture of culture medium and fetal bovine serum, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

Cell culture tested DMSO:ATCC 4-X

References: 55705: Sasaki H, et al. Serum level of the periostin, a homologue of an insect cell adhesion molecule, in thymoma patients. Cancer Lett. 172: 37-42, 2001. PubMed: 11595127

56105: Sasaki H, et al. 5H8細(xì)胞Serum level of the periostin, a homologue of an insect cell adhesion molecule, as a prognostic marker in nonsmall cell lung carcinomas. Cancer 92: 843-848, 2001. PubMed: 11550156