2Hx-2細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG2a
ATCC Number： HB-8117?
運(yùn)輸方式： 凍存運(yùn)輸
Designations: 2Hx-2
Depositors: Massachusetts Institute of Technology
2Hx-2細(xì)胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against adenovirus group specific antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with Hela cells that were infected with adenovirus 2.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
2Hx-2細(xì)胞The antibody reacts with adenoviruses of human subgroups A through E as well as with simian, bovine, canine and swine adenoviruses.
It does not react with avian adenoviruses.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 2Hx-2細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1197: Cepko CL, et al. Immunoprecipitation with two-dimensional pools as a hybridoma screening technique: Production and characterization of monoclonal antibodies against adenovirus 2 proteins. Virology 110: 385-401, 1981. PubMed: 7222481
3554: Cepko CL, et al. Production and use of monoclonal antibodies against adenoviruses. US Patent 4,487,829 dated Dec 11 1984
22704: Cepko CL, et al. Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J. Clin. Microbiol. 17: 360-364, 1983. 2Hx-2細(xì)胞PubMed: 6339552
