ECC-1 [EnCA1]細(xì)胞

器官來源： 其他

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2923?

是否是腫瘤細(xì)胞： 0

物種來源： 人

組織來源： luminal epithelium

Designations: ECC-1 [EnCA1]

Depositors: BA Lessey

ECC-1 [EnCA1]細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: endometrium adenocarcinoma

Tissue: luminal epithelium

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Receptors: androgen receptor, expressed

estrogen receptor 1 (ER alpha), expressed

estrogen receptor 2 (ER beta), expressed

progesterone receptor A, expressed

progesterone receptor B, expressed

interferon-gamma (IFN-gamma), expressed

ECC-1 [EnCA1]細(xì)胞Antigen Expression: cytokeratin-18; Homo sapiens, expressed

cytokeratin-13; Homo sapiens, expressed

epithelial membrane antigen; Homo sapiens, expressed

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9,12

D16S539: 9

D5S818: 10,11

D7S820: 9,10

THO1: 9,10

TPOX: 8

vWA: 14,17

Comments: EEC-1 was developed by Satyaswaroop by passing a well differentiated adenocarcinoma of human endometrial lining into nude mice. [PubMed; 6849115].The cells exhibit Epithelial Growth Factor (EGF-)-stimulated expression of known luminal proteins. This cell line can be used to study endocrine and paracrine regulation of endometrial epithelium in vitro. [PubMed: 16707768].

Propagation: ECC-1 [EnCA1]細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.

ECC-1 [EnCA1]細(xì)胞Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 6 X 10(3) to 1 X 10(4) viable cells/sq. cm is recommended.

Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 2 X 10(5) and 5 X 10(5) cells/sq. cm.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium,95%;DMSO,5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: about 26 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402

References: 92849: Mo B, et al. ECC-1 [EnCA1]細(xì)胞ECC-1 cells: a well-differentiated steroid-responsive endometrial cell line with characteristics of luminal epithelium. Biol. Reprod. 75: 387-394, 2006. PubMed: 16707768

92850: Satyaswaroop PG, et al. Human endometrial adenocarcinoma transplanted into nude mice: growth regulation by estradiol. Science 219: 58-60, 1983. PubMed: 6849115

92851: Tabibzadeh S, et al. Human endometrial epithelial cell lines for studying steroid and cytokine actions. In Vitro Cell. Dev. Biol. 26: 1173-1179, 1990. PubMed: 1706699

92852: Ricci MS, et al. ECC-1 human endometrial cells as a model system to study dioxin disruption of steroid hormone function. In Vitro Cell. Dev. Biol. Anim. 35: 183-189, 1999. PubMed: 10478797

92853: Castro-Rivera E, et al. Estrogen and aryl hydrocarbon responsiveness of ECC-1 endometrial cancer cells. Mol. Cell. Endocrinol. 150: 11-21, 1999. PubMed: 10411295