UMR-108細(xì)胞

器官來源： 骨

細(xì)胞形態(tài)： 成纖維樣

ATCC Number： CRL-1663?

運輸方式： 凍存運輸

相關(guān)**： 骨肉瘤

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

生長狀態(tài)： 貼壁生長

品系： Sprague-Dawley

Designations: UMR-108

Depositors: AE Bogden

UMR-108細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: fibroblast

Source: Organ: bone

Strain: Sprague-Dawley

Disease: osteosarcoma

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Receptors: parathyroid hormone (PTH); 1-25(OH)2D3 (bone resorbing steroid hormone)

Comments: UMR-108細(xì)胞The UMR-108 cell line is a clonal derivative of a transplantable rat osteosarcoma that had been induced by injection of radiophosphorous (32P).

The cells are responsive to PTH, prostaglandins and bone resorbing steroids.

The PTH responsiveness of UMR-106 is less than that of the related cell line UMR-106 (ATCC CRL-1661).

Both the original sarcoma and the cloned line were developed by T.J. Martin at the University of Sheffield.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and let the culture stand at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

References: 21887: . Endocrinology of calcium metabolism. Amsterdam: Elsevier/North Holland; 1977.

22331: Atkins D, et al. Rat osteogenic sarcoma cells: modulation of hormone stimulated cyclic AMP production by prostaglandin antagonists and biosynthesis inhibitors. Clin. Exp. Pharmacol. Physiol. 7: 31-44, 1980. PubMed: 6103768

22684: Manolagas SC, Deftos LJ. Cytoreceptor assay for 1,25-dihydroxyvitamin D3: a novel radiometric method based on binding of the hormone to intracellular receptors in vitro. Lancet 2: 401-402, 1980. PubMed: 6105521

22771: Partridge NC, et al. Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Lett. 115: 139-142, 1980. PubMed: 6248375

22847: Ingleton PM, et al. Alkaline phosphatase in serum and tumour of rats bearing a hormone- responsive transplantable osteogenic sarcoma. Eur. J. Cancer 15: 685-691, 1979. UMR-108細(xì)胞PubMed: 292595

22873: Crawford A, et al. Membranes from a transplantable osteogenic sarcoma responsive to parathyroid hormone and prostaglandins: regulation of adenylate cyclase and of hormone metabolism. J. Endocrinol. 77: 213-224, 1978. PubMed: 275436

22874: Atkins D, Martin TJ. Rat osteogenic sarcoma cells:effects of some prostaglandins, their metabolites and analogues on cyclic AMP production. Prostaglandins 13: 861-871, 1977. PubMed: 194286

22898: Martin TJ, et al. Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 260: 436-438, 1976. PubMed: 1062678

22974: Ingleton PM, et al. Radiation induced osteogenic sarcoma in the rat as a model of hormone-responsive differentiated cancer. Lab. Anim. Sci. 27: 748-756, 1977. PubMed: 338978

22975: Atkins D, et al. Rat osteogenic sarcoma cells: isolation and effects of hormones on the production of cyclic AMP and cyclic GMP. Endocrinology 101: 555-562, 1977. PubMed: 195798

22989: Crawford A, et al. Rat osteogenic sarcoma cells: comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin), 6-keto F1alpha and thromboxane B2 on cyclic AMP production and adenylate cyclase activity. Biochem. Biophys. Res. Commun. 82: 1195-1201, 1978. PubMed: 212039

23381: . . Clin. Orthop. Relat. Res. 140: 247-254, 1979.

23398: Manolagas SC, et al. 1,25-Dihydroxyvitamin D3 receptor-like macromolecule in rat osteogenic sarcoma cell lines. J. Biol. Chem. 255: 4414-4417, 1980. PubMed: 6929275

23491: Underwood JC, et al. Structural and functional correlations in parathyroid hormone responsive transplantable osteogenic sarcomas. Eur. J. Cancer 15: 1151-1158, 1979. UMR-108細(xì)胞PubMed: 294355

24392: . . Trans. Biochem. Sic. 6: 239-241, 1978.

26135: Partridge NC, et al. Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. Cancer Res. 43: 4308-4315, 1983. PubMed: 6575864

26136: Martin TJ, et al. Hormonal influences on bone cells. Methods Enzymol. 145: 324-336, 1987. PubMed: 3474491

26137: Forrest SM, et al. Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin. Calcif. Tissue Int. 37: 51-56, 1985. PubMed: 3922597