MDCK (NBL-2)細胞

運輸方式： 凍存運輸

器官來源： 腎臟

ATCC Number： CCL-34?

相關(guān)**： 正常

年限： *****

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 上皮樣

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 狗

Designations: MDCK (NBL-2)

MDCK (NBL-2)細胞Depositors: S Madin, NB Darby

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Canis familiaris

Morphology: epithelial

Source: Organ: kidney

Disease: normal

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: September, 1958

Applications:MDCK (NBL-2)細胞 transfection host

Virus Susceptibility: Human Coxsackievirus B 5

Reovirus type 2

Adeno-associated virus 4

Vaccinia virus

Vesicular stomatitis virus

Adeno-associated virus 5

Human Coxsackievirus B 3

Human Coxsackievirus B 4

Human poliovirus 2

Cytogenetic Analysis: Polyploidy 0.2%. Two large submetacentric chromosomes noted, presumably X chromosomes, and one or two additional chromosomes with median or submedian centromeres.

Age: *****

Gender: female

Comments: The MDCK cell line was derived from a kidney of an apparently normal ***** female cocker spaniel, September, 1958, by S.H. Madin and N.B. Darby. MDCK (NBL-2)細胞The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Rinse the cell layer twice with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 18385: Didier ES, et al. MDCK (NBL-2)細胞Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

22808: Haass C, et al. Polarized sorting of beta-amyloid precursor protein and its proteolytic products in MDCK cells is regulated by two independent signals. J. Cell Biol. 128: 537-547, 1995. PubMed: 7860629

25972: Gaush CR, et al. Characterization of an established line of canine kidney cells (MDCK). Proc. Soc. Exp. Biol. Med. 122: 931-935, 1966. PubMed: 5918973

28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

32843: Mead JR, et al. In vitro expression of mRNA coding for a Cryptosporidium parvum oocyst wall protein. J. Eukaryot. Microbiol. 43: 84-85, 1996. PubMed: 8822876

32899: von Dippe P, et al. The functional expression of sodium-dependent bile acid transport in Madin-Darby canine kidney cells transfected with the cDNA for microsomal epoxide hydrolase. J. Biol. Chem. 271: 18176-18180, 1996. PubMed: 8663355

33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

33080: Stuart RO, et al. Dependence of epithelial intercellular junction biogenesis on thapsigargin-sensitive intracellular calcium stores. J. Biol. Chem. 271: 13636-13641, 1996. PubMed: 8662885

33127: Grindstaff KK, et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996. PubMed: 8798517