MC-SV-HUC T-2細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來(lái)源： 輸尿管

ATCC Number： CRL-9519?

運(yùn)輸方式： 凍存運(yùn)輸

年限： 11 years

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

組織來(lái)源： uroepithelium

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

Designations: MC-SV-HUC T-2

MC-SV-HUC T-2細(xì)胞Depositors: Wisconsin Alumni Res. Fndn.

Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: ureter

Tissue: uroepithelium

Cellular Products: uroepithelial keratins

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Tumorigenic: MC-SV-HUC T-2細(xì)胞Yes

Age: 11 years

Gender: male

Comments: The cells were initially transformed with SV40 virus, and later treated with 3-methylcholanthrene (MCA).

While the parental line (SV-HUC-1, see ATCC CRL-9520) was not tumorigenic and had a balanced chromosome composition, the MCA treated line became tumorigenic and aneuploid.

The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.

Stress (such as chemical exposure) could possibly activate the virus.

Propagation:MC-SV-HUC T-2細(xì)胞 ATCC complete growth medium: Ham's F12 medium, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. MC-SV-HUC T-2細(xì)胞US Patent 4,980,290 dated Dec 25 1990