Ep-16細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： HB-155?

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgM; kappa light chain

Designations: Ep-16

Depositors: AW Hamburger

Isotype: IgM; kappa light chain

Ep-16細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human epithelium

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Ep-16細(xì)胞Animals were immunized with a membrane enriched extract of human foreskin keratinocytes.

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with an epithelial specific antigen on human foreskin keratinocytes but does not react with mesodermal cells or lymphocytes.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Ep-16細(xì)胞Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

References: 1003: Hamburger AW, et al. Isolation and characterization of a monoclonal antibody specific for epithelial cells. Cancer Res. 45: 783-790, 1985. Ep-16細(xì)胞PubMed: 2578308