J558細(xì)胞

細(xì)胞類(lèi)型： B**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgA; lambda light chain

數(shù)量： 大量

品系： BALB/c

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： TIB-6?

相關(guān)**： 多發(fā)性骨髓瘤

Designations: J558

J558細(xì)胞Depositors: M Cohn

Isotype: IgA; lambda light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: plasmacytoma; myeloma

Strain: BALB/c

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against alpha-1,3-dextran

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Antigen Expression: J558細(xì)胞H-2d; PC.1

Cytogenetic Analysis: diploid

Comments: The plasmacytoma was induced with mineral oil in a BALB/c mouse.

The cells are sensitive to dexamethasone and cortisol.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: J558細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2040

References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956

22228: Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326

22522: Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

22586: Carson D, Weigert M. Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody. Proc. Natl. Acad. Sci. USA 70: 235-239, 1973. PubMed: 4119224

22744: Lundblad A, et al. Immunochemical studies on mouse myeloma proteins with specificity for dextran or for levan. Immunochemistry 9: 535-544, 1972. PubMed: 5036150

23254: Cesari IM, Weigert M. Mouse lambda-chain sequences. Proc. Natl. Acad. Sci. USA 70: 2112-2116, 1973. PubMed: 4516208

23494: Weigert MG, et al. J558細(xì)胞Variability in the lambda light chain sequences of mouse antibody. Nature 228: 1045-1047, 1970. PubMed: 5483159

26330: . . Science 177: 1972.