CCD-1068Sk細胞

生長狀態(tài)： 貼壁生長

器官來源： 皮膚

細胞形態(tài)： 成纖維樣

細胞類型： 成纖維細胞

是否是腫瘤細胞： 0

物種來源： 人

運輸方式： 凍存運輸

年限： 65 years

ATCC Number： CRL-2086?

CCD-1068Sk細胞相關(guān)**： 正常

數(shù)量： 大量

Designations: CCD-1068Sk

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. CCD-1068Sk細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 65 years

Gender: female

Comments: The line was established from skin taken from normal breast tissue removed at mastectomy for carcinoma of the right breast.

Cells senesce after approximately 33 population doublings.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. CCD-1068Sk細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. CCD-1068Sk細胞Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; DMSO, 5%