QPN1 12C9 (SCRF 43.2)細胞
生長狀態(tài)： 懸浮生長
ATCC Number： HB-9500?
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
數(shù)量： 大量
細胞形態(tài)： **樣
**類型： IgG1
Designations: QPN1 12C9 (SCRF 43.2)
QPN1 12C9 (SCRF 43.2)細胞Depositors: Scripps Clinic and Research Foundation
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against p-nitroanaline amide derivatives
Permits/Forms: QPN1 12C9 (SCRF 43.2)細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with a conjugate of keyhole limpet hemocyanin and a phosphonamidate compound.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with and catalyzes the hydrolysis of certain ester bonds.
Propagation: QPN1 12C9 (SCRF 43.2)細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with:10 mM HEPES0.1 mM hypoxanthine0.032 mM thymidine2% bovine serum albumin1% Nutridoma-SP (Boehringer Mannheim Biochemicals, Catalog# 100-104)
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 21999: Lerner RA, et al. QPN1 12C9 (SCRF 43.2)細胞Molecules with antibody combining sites that exhibit catalytic properties. US Patent 5,126,258 dated Jun 30 1992
