34-4-21S細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgM

ATCC Number： HB-76?

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 34-4-21S

Depositors: DH Sachs, K Ozato

Isotype: IgM

34-4-21S細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against H-2 D d; cytotoxic

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Comments: 34-4-21S細(xì)胞Animals were immunized with spleen cells from BDF1.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with cells from strains that have the H-2d haplotype either in the H-2D region or in the entire major histocompatibility complex.

A 20 fold concentration of supernatant may be necessary to detect the antibody by immunodiffusion.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

34-4-21S細(xì)胞fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 58003: Ozato K, et al. Monoclonal antibodies to mouse MHC antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. 34-4-21S細(xì)胞Transplantation 34: 113-118, 1982. PubMed: 7135466