QNR/K2細胞

ATCC Number： CRL-2533?

細胞形態(tài)： 混合型

數(shù)量： 大量

年限： embryo, 7 days gestation

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 其他

運輸方式： 凍存運輸

組織來源： neuroretina

Designations: QNR/K2

Depositors: B Pessac, D Trisler

QNR/K2細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Coturnix coturnix japonica

Morphology: mixed

Source: Tissue: neuroretina

Cell Type: infected with Rous sarcoma virus mutant ts NY-68

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Age: embryo, 7 days gestation

Comments: Neuroretinas were dissected from normal quail embryos, dissociated and immortalized by infection with Rous sarcoma virus (RSV) mutant ts NY-68 to establish NR cell lines. [48962]

Cells were subsequently cloned after 18 months in culture to establish the cloned cell line QNR/K2. [48962]

Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; QNR/K2細胞and ganglion neuronal cells. [48961]

The QNR/K2 cell line displays properties of Muller (astroglia) cells.

The cells were able to respond to electrical stimulation by producing action potentials which were inhibited by 10(-7) M tetrodotoxin. [48962]

The specific activity of glutamic acid decarboxylase (GAD), the enzyme responsible for the synthesis of the neurotransmitter gamma-aminobutyric acid, was high. [48962]

QNR/D cells (ATCC CRL-2532) and QNR/K2 cell (ATCC CRL-2533) were transplanted into chicken embryo eyes. Implanted QNR/D cells migrate only to the retinal ganglion and amacrine cell layers and project neurites in the plane of retina. [48964]

In contrast, QNR/K2 cells migrate through the ganglion and amacrine layers, locate in the inner nuclear layer, and project processes across the retina. [48964]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 39.0°C

Subculturing: QNR/K2細胞Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 48961: Pessac B, et al. A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture. Nature 302: 616-618, 1983. PubMed: 6300691

48962: Pessac B, et al. Cells with neuronal properties in permanent cultures of quail embryo neuroretinas infected with Rous sarcoma virus. Brain Res. 275: 53-59, 1983. PubMed: 6313126

48964: Trisler D, et al. Retinal engineering: QNR/K2細胞engrafted neural cell lines locate in appropriate layers. Proc. Natl. Acad. Sci. USA 93: 6269-6274, 1996. PubMed: 8692804