RPMI 7666細胞

年限： 32 years

ATCC Number： CCL-114?

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

器官來源： 外周血

運輸方式： 凍存運輸

細胞形態(tài)： **樣

細胞類型： B**細胞

是否是腫瘤細胞： 0

物種來源： 人

Designations: RPMI 7666 [Gerner 7666]

Depositors: GE Moore

RPMI 7666細胞Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: B lymphoblast;

Cellular Products: immunoglobulin; Epstein-Barr virus (EBV)

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Antigen Expression: HLA A3, Aw29, B7, B12; Blood type B+

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 8,12

D16S539: 9,11

D5S818: 11,12

D7S820: 8,11

THO1: 7,9.3

TPOX: 8,11

vWA: 14,16

Age: 32 years

Gender: male

RPMI 7666細胞Ethnicity: Caucasian

Comments: Biological Safety CLASS II.

EBNA positive.

The cells produce Epstein-Barr virus.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml.

References: 22312: Moore GE, et al. Culture of normal human leukocytes. RPMI 7666細胞JAMA 199: 519-524, 1967. PubMed: 4960081