30-C7細(xì)胞

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

ATCC Number： TIB-106?

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG2a

Designations: 30-C7

Depositors: JA Ledbetter

Isotype: IgG2a

30-C7細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; 30-C7細(xì)胞monoclonal antibody; against Lgp100a (a 100000 dalton glycoprotein that is expressed on mouse T and B cells)

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Comments: Animals were immunized with mouse splenic or thymic lymphocytes.

Spleen cells were fused with NS-1 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 30-C7細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

References: 58000: Ledbetter JA, Herzenberg LA. 30-C7細(xì)胞Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol. Rev. 47: 63-90, 1979. PubMed: 398327