QPN1 22F5 (SCRF 43.2)細胞

運輸方式： 凍存運輸

細胞形態(tài)： **樣

**類型： IgG1

ATCC Number： HB-9509?

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

Designations: QPN1 22F5 (SCRF 43.2)

QPN1 22F5 (SCRF 43.2)細胞Depositors: Scripps Clinic and Research Foundation

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: QPN1 22F5 (SCRF 43.2)細胞Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against p-nitroanaline amide derivatives

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a conjugate of keyhole limpet hemocyanin and a phosphonamidate compound.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

QPN1 22F5 (SCRF 43.2)細胞The antibody reacts with and catalyzes the hydrolysis of certain ester bonds.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 1 mM sodium pyruvate, 1 mM sodium oxaloacetate, 0.1 mM hypoxanthine, 0.032 mM thymidine; penicillin, streptomycin and gentamicin, 97%; bovine serum albumin,

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 21999: Lerner RA, et al. QPN1 22F5 (SCRF 43.2)細胞Molecules with antibody combining sites that exhibit catalytic properties. US Patent 5,126,258 dated Jun 30 1992