3197-3 (3T3-MSV)細胞

運輸方式： 凍存運輸

器官來源： 胚胎

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 成纖維樣

細胞類型： 成纖維細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

數(shù)量： 大量

3197-3 (3T3-MSV)細胞年限： embryo

ATCC Number： CRL-1568?

Designations: 3197-3 (3T3-MSV)

Depositors: JE DeLarco

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: embryo

Cell Type: fibroblast

Cellular Products: 3197-3 (3T3-MSV)細胞The cells release a growth factor in serum free medium.

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: embryo

Comments: The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.

They do not produce virus particles.

The cells have a tendency to slough off the flask surface.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 3197-3 (3T3-MSV)細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 95%; bovine calf serum, 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended

Medium Renewal: 1 to 2 times per week

Remove medium, add fresh 0.25% trypsin solution for 5 minutes, add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days.

References: 23261: de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512

26089: Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. 3197-3 (3T3-MSV)細胞PubMed: 4319233