3-83P細(xì)胞
ATCC Number： HB-20?
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG2a; kappa light chain
數(shù)量： 大量
運輸方式： 凍存運輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
Designations: 3-83P
3-83P細(xì)胞Depositors: DH Sachs, K Ozato
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against H-2 K k, D k; cytotoxic
Permits/Forms: 3-83P細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with spleen cells from C3H mice.
Lymph node cells were fused with P3X63Ag8 myeloma cells.
The antibody cross-reacts with H-2Kb, H-2s, H-2r, H-2p and H-2q.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. 3-83P細(xì)胞To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 905: Ozato K, et al. 3-83P細(xì)胞Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699
