ZFL [ZF-L]細胞

細胞形態(tài)： 上皮樣

運輸方式： 凍存運輸

數(shù)量： 大量

器官來源： 肝

ATCC Number： CRL-2643?

相關**： 正常

生長狀態(tài)： 貼壁生長

年限： *****

是否是腫瘤細胞： 0

物種來源： 斑馬魚

ZFL [ZF-L]細胞Designations: ZFL [ZF-L]

Depositors: DW Barnes

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Danio rerio deposited as zebrafish

Morphology: epithelial

Source: Organ: liver

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. ZFL [ZF-L]細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1992

Cytogenetic Analysis: hypodiploid; modal number = 47

Age: *****

Comments: The cells synthesize and release several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG. [53447]

ZFL cell homogenates exhibit alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. [53447]

The dioxin, TCDD, induces one or possibly two forms of cytochrome P450 immunochemically and functionally related to rainbow trout P4501A1. [53448]

The cells are useful for studies of liver cell metabolism and xenobiotic formation. They may be used for in vitro toxicology studies.

A culture submitted to the ATCC in August of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.

The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

Propagation: ZFL [ZF-L]細胞ATCC complete growth medium: These cells are grown in

50 % L-15 (ATCC 30-2008)

35 % DMEM HG (GIBCO 12100)

15 % Ham's F12 (GIBCO 21700)

All without sodium bicarbonate Supplemented with:

0.15 g/L sodium bicarbonate

15 mM HEPES

0.01mg/ml bovine insulin

50 ng/ml mouse EGF

5% heat-inactivated fetal bovine serum

0.5% Trout Serum

Do not filter complete medium.

Atmosphere: air, 100%

Temperature: 28.0°C; (Max. 29.0 °C, Min. 26.0°C)

Subculturing: ZFL [ZF-L]細胞Protocol: Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture to sit at room temperature (or 28C) until the cells detach (about 30 minutes). Add fresh medium, and centrifuge at 1000 rpm for 10 minutes. Resuspend the pellet in fresh serum-free growth medium and dispense into new flasks. Incubate at 28C for 30 minutes. Examine to ensure attachment and then add heat-inactivated FBS for a final concentration of 5% and Trout serum for a final concentration of 0.5%.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Preservation: Freeze medium: Culture medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 5% DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 72 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

References: 53447: Ghosh C, et al. Derivation and characterization of a zebrafish liver cell line. Cell Biol. Toxicol. 10: 167-176, 1994. PubMed: 7994634

53448: Miranda CL, et al. Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio). Arch. Biochem. Biophys. 305: 320-327, 1993. ZFL [ZF-L]細胞PubMed: 8373170

53449: Collodi P, et al. Induction of zebrafish (Brachydanio rerio) P450 in vivo and in cell culture. Xenobiotica 24: 487-493, 1994. PubMed: 7975714