3C11細胞
生長狀態(tài)： 懸浮生長
是否是腫瘤細胞： 0
物種來源： 小鼠
數(shù)量： 大量
ATCC Number： HB-8511?
運輸方式： 凍存運輸
細胞形態(tài)： **樣
**類型： IgG1; kappa light chain
Designations: 3C11
3C11細胞Depositors: Temple University
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:3C11細胞 immunoglobulin; monoclonal antibody; against bacterial cell wall peptidoglycan
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with Streptococcus mutans (BHT strain) bacterial cell wall peptidoglycan.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with cell wall peptidoglycans from a variety of gram- and gram+ organisms.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. 3C11細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 3549: Jackson DE, et al. 3C11細胞Monoclonal antibodies to peptidoglycan and methods of preparing same. US Patent 4,596,769 dated Jun 24 1986
