372細胞
數(shù)量： 大量
細胞形態(tài)： **樣
**類型： IgG3
ATCC Number： CRL-1893?
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
Designations: 372
372細胞Depositors: B Chesebro
Isotype: IgG3
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 372細胞immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
The antibody is highly specific for p15E protein from most MuLV.
The antibody efficiently immunoprecipitates p15E and works well in Western blotting assays for p15E.
Propagation: 372細胞ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1778: Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798
1779: Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. 372細胞PubMed: 6305011
