Rn2T細胞

是否是腫瘤細胞： 0

物種來源： 褐鼠

器官來源： 乳腺

數(shù)量： 大量

ATCC Number： CRL-6599?

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

年限： 2 years

Designations: Rn2T

Rn2T細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus

Morphology:

Source: Organ: mammary gland

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Age: 2 years

Gender: female

Comments: Rn2T細胞Spontaneous tumors in a pet rat.

Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Rn2T細胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended mediumRn2T細胞 (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020