3C10細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG2b

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： TIB-228?

Designations: 3C10

Depositors: RM Steinman

3C10細(xì)胞Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human monocyte; against CD14; cytotoxic

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Tumorigenic: Yes

Comments: Animals were immunized with human peripheral blood monocytes.

Spleen cells were fused with P3X63Ag8-U1 myeloma cells.

The antibody reacts with human monocytes but not with platelets, lymphocytes, dendritic cells, epithelial cells or fibroblasts.

The antibody appears to react with all tissue macrophages, and to differentiate between macrophages and dendritic cells.

The antibody the antibody immunoprecipitates gp55.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 3C10細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1194: Van Voorhis WC, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J. Exp. Med. 158: 126-145, 1983. PubMed: 6190974

1195: Van Voorhis WC, et al. Relative efficacy of human monocytes and dendritic cells as accessory cells for T cell replication. J. Exp. Med. 158: 174-191, 1983. PubMed: 6190976

1318: Zvaifler NJ, et al. 3C10細(xì)胞Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis. J. Clin. Invest. 76: 789-800, 1985. PubMed: 3875632