36F-18C [36F]細(xì)胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

ATCC Number： HB-285?

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgG2a

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: 36F-18C [36F]

36F-18C [36F]細(xì)胞Depositors: CR Mackay

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against sheep (ovine) CD2

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 36F-18C [36F]細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with phytohemagglutinin-stimulated sheep T cells.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes.

36F-18C [36F]細(xì)胞It does not inhibit cytotoxic T lymphocyte mediated killing or mixed lymphocyte reactivity.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23177: Mackay CR, et al. 36F-18C [36F]細(xì)胞Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18: 1681-1688, 1988. PubMed: 2462499