Ro Bon細胞

年限： 21 years

是否是腫瘤細胞： 0

物種來源： 人

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 成纖維樣

ATCC Number： CRL-1248?

相關(guān)**： 成骨不全癥

數(shù)量： 大量

器官來源： 皮膚

運輸方式： 凍存運輸

Ro Bon細胞Designations: Ro Bon

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: osteogenesis imperfecta (tarda)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Ro Bon細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 21 years

Gender: female

Comments: The tissue donor suffered from osteogenesis imperfecta (tarda), and was the mother of Da Bon (ATCC CRL-1251).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Ro Bon細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.Ro Bon細胞 Subculture every 5 to 7 days.