ESK-4細胞

是否是腫瘤細胞： 0

物種來源： 豬

年限： embryo

數(shù)量： 大量

ATCC Number： CL-184?

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

器官來源： 腎臟

Designations: ESK-4

Depositors: Syntex (USA), Inc.

ESK-4細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Sus scrofa

Morphology:

Source: Organ: kidney

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Susceptibility: Bovine viral diarrhea virus 1

Cytogenetic Analysis: normal female; diploid

Age: ESK-4細胞embryo

Gender: female

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA, remove trypsin and let the flask sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

ESK-4細胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Storage temperature: liquid nitrogen vapor phase

References: 3523: Bordt DE, Thomas PC. Diploid porcine embryonic cell strains, cultures produced therefrom, and use of said cultures for production of vaccines. ESK-4細胞US Patent 4,070,453 dated Jan 24 1978