Ro Dow細胞

是否是腫瘤細胞： 0

物種來源： 人

運輸方式： 凍存運輸

器官來源： 皮膚

數量： 大量

細胞形態(tài)： 成纖維樣

年限： 65 years

ATCC Number： CRL-1337?

相關**： 其他**

生長狀態(tài)： 貼壁生長

Designations: Ro Dow

Ro Dow細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Ro Dow細胞Disease: Ehlers-Danlos syndrome, type II

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 65 years

Gender: female

Comments: Ro Dow細胞The tissue donor suffered from type II Ehlers-Danlos syndrome.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Ro Dow細胞Subculture every 10 to 14 days.