3C5.1細(xì)胞
ATCC Number： CRL-2284?
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
**類型： IgG2a
生長狀態(tài)： 懸浮生長
3C5.1細(xì)胞Designations: 3C5.1
Depositors: S Linn
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:3C5.1細(xì)胞 immunoglobulin; monoclonal antibody; against human DNA polymerase epsilon (pol epsilon)
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Comments: Animals were immunized with Mono S FPLC purified DNA polymerase epsilon.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon.
It does not recognize DNA polymerases alpha, beta or delta.
3C5.1細(xì)胞The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM hypoxanthine and 0.008 mM thymidine, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 24404: 3C5.1細(xì)胞Chui G, Linn S. Further characterization of HeLa DNA polymerase epsilon. J. Biol. Chem. 270: 7799-7808, 1995. PubMed: 7536191
