3B-11細(xì)胞

年限： *****

器官來(lái)源： 腋窩**結(jié)

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

品系： C3H/HeJ

組織來(lái)源： vascular epithelium

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類(lèi)型： SV40轉(zhuǎn)化細(xì)胞

ATCC Number： CRL-2160?

是否是腫瘤細(xì)胞： 0

3B-11細(xì)胞物種來(lái)源： 小鼠

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

Designations: 3B-11

Depositors: KA O'Connell

Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: axillary lymph node

Strain: C3H/HeJ

Tissue: 3B-11細(xì)胞vascular epithelium

Cell Type: endothelialSV40 transformed

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1992

Tumorigenic: Yes

Antigen Expression: H-2 K; VCAM

Age: *****

Gender: male

Comments: The 3B-11 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).

3B-11 cells were cloned in 1992 by limiting dilution.

They are resistant to lysis by activated macrophages as measured in the chromium release assay.

This clone retains the ability to differentiate on a synthetic basement-like membrane.

The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).

The cells stain positively for SV40 T antigen.

Propagation: 3B-11細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: 3B-11細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

23187: O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299