3T6-Swiss albino細胞
運輸方式： 凍存運輸
細胞類型： 成纖維細胞
細胞形態(tài)： 成纖維樣
是否是腫瘤細胞： 0
物種來源： 小鼠
年限： embryo
生長狀態(tài)： 貼壁生長
數(shù)量： 大量
ATCC Number： CCL-96?
3T6-Swiss albino細胞器官來源： 胚胎
Designations: 3T6-Swiss albino
Depositors: H Green
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Organ: embryo
Cell Type: fibroblast
Cellular Products: 3T6-Swiss albino細胞collagen; hyaluronic acid
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Applications: transfection host
Virus Resistance: poliovirus 2
Cytogenetic Analysis: Karyotype unstable within stemline range (78-81). Approximately 21% of the cells contained an extra large chromosome with a terminal centromere and 21% contained minute chromosomes.
Age: embryo
Comments: The 3T6 cell line is a collagen and hyaluronic acid secreting line established by G. Todaro and H. Green in 1963 from disaggregated Swiss mouse embryos. [5732]
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: 3T6-Swiss albino細胞Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 5732: Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244
26261: Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482
26270: Green H, et al. 3T6-Swiss albino細胞Differentiated cell types and the regulation of collagen synthesis. Nature 212: 631-633, 1966. PubMed: 5971697
