EOC 2細胞

細胞類型： 其他細胞類型

品系： C3H/HeJ

數(shù)量： 大量

器官來源： 大腦

細胞形態(tài)： 單核細胞/巨噬細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

年限： 10 days

ATCC Number： CRL-2467?

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

EOC 2細胞Designations: EOC 2

Depositors: WS Walker

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus deposited as mouse

Morphology: macrophage

Source: Organ: brain

Strain: C3H/HeJ

Cell Type: microglia;

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Receptors: EOC 2細胞colony stimulating factor 1 (CSF-1R, CD115)

Antigen Expression: CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR + [39974]

Age: 10 days

Gender: female

Comments: This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. [39974]

Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping.

The cell line is dependent on colony stimulating factor 1 (CSF-1).

The cells exhibit phagocytic activity.

Unlike EOC 13.31 (ATCC CRL-2468) and EOC 20 (ATCC CRL-2469), these cells do not constitutively express high levels of major histocompatibility complex (MHC) class II antigens and expression is not upregulated by recombinant murine interferon-gamma.

The cells may be used to characterize the role of brain macrophages. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)

Propagation: EOC 2細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard 75% of the media.

Scrape off the attached cells with a cell scraper.

Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: EOC 2細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

39968: Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530

39974: Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814

39975: Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775