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產(chǎn)品資料

EOMA細(xì)胞

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產(chǎn)品名稱: EOMA細(xì)胞
產(chǎn)品型號(hào): EOMA
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

EOMA細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。EOMA細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


EOMA細(xì)胞  的詳細(xì)介紹

EOMA細(xì)胞

ATCC Number: CRL-2586?

相關(guān)**: 其他**

運(yùn)輸方式: 凍存運(yùn)輸

細(xì)胞形態(tài): 內(nèi)皮樣

數(shù)量: 大量

品系: 129

細(xì)胞類型: 內(nèi)皮細(xì)胞

是否是腫瘤細(xì)胞: 0

物種來源: 小鼠

生長狀態(tài): 貼壁生長

EOMA細(xì)胞年限: *****

Designations: EOMA

Depositors: R Auerbach

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus deposited as mouse

Morphology: endothelial


Source: Disease: hemangioendothelioma

Strain: 129

Cell Type: endothelial

Cellular Products: angiotensin converting enzyme (ACE) [51514]

thrombospondin [51514]

cathepsin L [47148]

endostatin [47148]

interleukin-6 (interleukin 6, IL-6)EOMA細(xì)胞 [53484]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1980

Receptors: acetylated low density liproprotein [51514]

Tumorigenic: Yes

Antigen Expression: CD31 +

vascular addressin +

CD45 (Ly5-T200) +

Age: *****

Comments: The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an ***** mouse. [51514]

EOMA細(xì)胞The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514]

Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148]

Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). [53484]

The cells constitutively express the vascular addressin identified by antibody MECA-99.

EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.


Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: EOMA細(xì)胞Complete growth medium supplemented with 5% DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

derivative:ATCC CRL-2587

References: 47148: Felbor U, et al. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 19: 1187-1194, 2000. PubMed: 10716919

51514: Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185

53484: Zhu XL, et al. Kinetics of cytokine gene expression in macrophage and endothelial cell lines following liposome encapsulated haemoglobin (LEH) treatment in vitro. Cytokine 8: 541-547, 1996. PubMed: 8891435

53486: Wen W, et al. The generation of endostatin is mediated by elastase.. Cancer Res. 59: 6052-6056, 1999. PubMed: 10626789

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