RLSD06細胞

ATCC Number： HB-8527?

細胞形態(tài)： **樣

**類型： IgG1

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 小鼠

Designations: RLSD06 [Anti-PA 1, DB90 5D5.4.3]

RLSD06細胞Depositors: Cetus Corp.

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human prostate antigen (PA, 33000 dalton glycoprotein)

Permits/Forms: RLSD06細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with purified PA from human semen.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Propagation: RLSD06細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: fetal bovine serum, 90%; DMSO, 10%

References: 3896: M'Timkulu T, et al. RLSD06細胞Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay. US Patent 4,690,890 dated Sep 1 1987