3H5-1細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

ATCC Number： HB-46?

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

細(xì)胞形態(tài)： **樣

**類型： IgG1

Designations: 3H5-1

3H5-1細(xì)胞Depositors: MK Gentry

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a type specific determinant on Dengue virus-2)

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Tumorigenic: Yes

Comments: Animals were immunized with dengue virus type 2 antigens from infected mouse brains (the prototype strain New Guinea C was used).

The antibody is type specific.

Spleen cells were fused with P3X63Ag8 myeloma cells.

Antibodies should be prepared as ascites in pristane primed BALB/c mice.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 3H5-1細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 922: Henchal EA, et al. 3H5-1細(xì)胞Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749