39-S細(xì)胞

ATCC Number： HB-8180?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG2a

生長狀態(tài)： 懸浮生長

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: 39-S

39-S細(xì)胞Depositors: The United States of America

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) internal capsid protein 8 (ICP 8)

Permits/Forms: 39-S細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with HSV-1.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

The antibody is type specific for HSV-1 but does not neutralize the virus.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15% -OR- RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: 39-S細(xì)胞Medium Renewal: Every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1178: Showalter SD, et al. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infect. Immun. 34: 684-692, 1981. PubMed: 6277788

3544: Zweig M, et al. Monoclonal antibodies to herpes simplex virus type 1 polypeptides. 39-S細(xì)胞US Patent 4,572,896 dated Feb 25 1986