SJD.1細(xì)胞

ATCC Number： CRL-2296?

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

是否是腫瘤細(xì)胞： 0

物種來源： 斑馬魚

器官來源： 其他

品系： SJD

年限： *****

運(yùn)輸方式： 凍存運(yùn)輸

Designations: SJD.1

SJD.1細(xì)胞Depositors: BH Paw, LI Zon

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Danio rerio

Morphology: fibroblast

Source: Organ: caudal fin

Strain: SJD

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: *****

Comments: SJD.1細(xì)胞SJD.1 is a fibroblast cell line derived from amputated caudal fins of an ***** zebrafish, strain SJD.

This strain of zebrafish is used for genetic mapping.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.

Temperature: 28.0°C

SJD.1細(xì)胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, rinse with trypsin (0.25%)-EDTA (0.03%)-polyvinylpyrrolidone (0.5%) solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature until cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: SJD.1細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 43315: Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354