EL4.IL-2細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

器官來(lái)源： 胸腺

ATCC Number： TIB-181?

相關(guān)**： **瘤

細(xì)胞形態(tài)： **樣

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

品系： C57BL/6

Designations: EL4.IL-2

EL4.IL-2細(xì)胞Depositors: JJ Farrar

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: thymus

Strain: C57BL/6

Disease: lymphoma

Cellular Products: interleukin-2 (interleukin 2, IL-2)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.EL4.IL-2細(xì)胞 Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: This is a subline of EL4 (ATCC TIB-39) that produces IL-2 in response to phorbol-12-myristate-13-acetate (PMA).

The line is capable of producing 2500 units/ml of IL-2 after 24 hours in culture with PMA.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 to 2 X 10(6) viable cells/ml..

EL4.IL-2細(xì)胞Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2040

parental cell line:ATCC TIB-39

References: 1142: Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789

21883: . Cancer therapeutics. New York: Raven Press; 1981.

23210: Fuller-Farrar J, et al. EL4.IL-2細(xì)胞Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931