EL4.BU.1.OUAr.1.1細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

品系： C57BL/6N

ATCC Number： TIB-41?

相關(guān)**： **瘤

細(xì)胞類型： T**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

細(xì)胞形態(tài)： **樣

EL4.BU.1.OUAr.1.1細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: EL4.BU.1.OUAr.1.1

Depositors: R Hyman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: C57BL/6N

Cell Type: EL4.BU.1.OUAr.1.1細(xì)胞T lymphocyte;

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Antigen Expression: H-2b; Thy-1.2

Comments: This line was derived from the EL4 cell line (see ATCC TIB-39).

The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain.

EL4.BU.1.OUAr.1.1細(xì)胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22247: Hyman R, Stallings V. EL4.BU.1.OUAr.1.1細(xì)胞Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.