SHM-D33細胞

細胞類型： 其他細胞類型

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 其他

數(shù)量： 大量

細胞形態(tài)： **樣

ATCC Number： CRL-1668?

組織來源： heteromyeloma

生長狀態(tài)： 懸浮生長

SHM-D33細胞Designations: SHM-D33

Depositors: HS Kaplan

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens; Mus musculus

Morphology: lymphoblast

Source: Tissue: heteromyeloma

Cell Type: SHM-D33細胞B lymphoblast; somatic cell hybrid

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Comments: This line was produced by fusing the human myeloma cell line FU-266, clone E-1 (HAT sensitive, 8-azaguanine resistant and resistant to G-418 - an antibiotic similar to gentamicin) with the murine myeloma P3X63Ag8.653 (see ATCC CRL-1580).

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Modified Dulbecco's medium supplemented with 0.2 mg/ml G418 (Geneticin) and 20% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: SHM-D33細胞The cells are grown in 200 to 400 mcg/ml of G-418 to maintain selection for the human chromosomes.

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4 X 10(4) viable cells/ml.

Interval: Maintain cultures at a cell concentraton between 3 X 10(4) and 8 X 10(5) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 992: Teng NN, et al. Construction and testing of mouse-human heteromyelomas for human monoclonal antibody production. Proc. Natl. Acad. Sci. USA 80: 7308-7312, 1983. PubMed: 6316357

1075: Kearney JF, et al. SHM-D33細胞A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548-1550, 1979. PubMed: 113458

1180: Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745