3Pt12B8細(xì)胞

生長狀態(tài)： 懸浮生長

ATCC Number： HB-8136?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgM

3Pt12B8細(xì)胞運輸方式： 凍存運輸

Designations: 3Pt12B8

Depositors: Sidney Farber Cancer Institute

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

3Pt12B8細(xì)胞Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with human T chronic lymphocytic leukemia cells.

Spleen cells were fused with NS-1 myeloma cells.

The antibody has been used to prevent graft versus host disease (GVHD).

Propagation: 3Pt12B8細(xì)胞ATCC complete growth medium: Modified Dulbecco's medium; horse serum, 10%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 3512: Reinherz EL, Schlossman SF. Monoclonal antibody. 3Pt12B8細(xì)胞US Patent 4,443,427 dated Apr 17 1984