3G9F3細胞

ATCC Number： CRL-1843?

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： IgG2b

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

運輸方式： 凍存運輸

Designations: 3G9F3

3G9F3細胞Depositors: CA Whetstone

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against pseudorabies virus (PRV)

Permits/Forms: 3G9F3細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with pseudorabies virus (IA strain 62-26).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

3G9F3細胞The antibody is specific for the gIII protein of PRV.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. 3G9F3細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.