EL4.BU細(xì)胞
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： TIB-40?
相關(guān)**： **瘤
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
品系： C57BL/6N
細(xì)胞形態(tài)： **樣
EL4.BU細(xì)胞Designations: EL4.BU
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6N
Cell Type: T lymphocyte;
Permits/Forms: EL4.BU細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2b; Thy-1.2
Comments: This line was derived from the EL4 cell line (see ATCC TIB-39).
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine (BUdR).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: EL4.BU細(xì)胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products: parental cell line:ATCC TIB-39
References: 1113: Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030
1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956
22173: Kohler G, et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123
22247: Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma.EL4.BU細(xì)胞 Immunogenetics 3: 75-84, 1976.
