SH4細胞

運輸方式： 凍存運輸

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

**類型： IgG1

生長狀態(tài)： 懸浮生長

ATCC Number： HB-10745?

SH4細胞Designations: SH4

Depositors: Osiris Therapeutics, Inc.

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: SH4細胞Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human mesenchymal stem cells

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Tumorigenic: Yes

Comments: Animals were immunized with human mesenchymal stem cells.

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

The antibody reacts with mesenchymal stem cells from bone marrow and some tissues of mesenchymal origin.

Propagation: SH4細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 20%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphereATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%

Storage temperature: liquid nitrogen vapor phase

Related Products: SH4細胞recommended serum:ATCC 30-2020

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996

49752: Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137