RHCT-138細(xì)胞

ATCC Number： CRL-2563?

器官來(lái)源： 皮膚

相關(guān)**： 其他**

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，多細(xì)胞聚集

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 其他

年限： 4.5 years

數(shù)量： 大量

RHCT-138細(xì)胞細(xì)胞形態(tài)： **樣

Designations: RHCT-138

Depositors: RM Simpson

Biosafety Level: 2 [Cells infected with HTLV-I virus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Oryctolagus cuniculus deposited as rabbit

Morphology: lymphoblast

Source: Organ: skin

Disease: cutaneous T cell lymphoma

Cell Type: infected with HTLV-I

Cellular Products: RHCT-138細(xì)胞HTLV-I (human T cell leukemia virus)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: 1. This cell line was deposited at the ATCC by Dr. R. Mark Simpson and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of these cells, or their products must first be negotiated with the National Institute of Allergy and Infectious Diseases (NIAID) Office of Technology Development (OTD), Building 31, Room 3B-62 31 Center Drive, MSC 2137 Bethesda, MD 20892-2137 TEL: 301-496-2644 FAX: 301-402-7123

Isolation: Isolation date: February, 1997

Age: 4.5 years

Gender: male

Comments: RHCT-138細(xì)胞The RHCT-138 cell line was derived from a skin biopsy taken from an HTLV-1 infected rabbit (X138) with cutaneous T cell lymphoma (CTCL). [50754]

The rabbit developed chronic HTLV-1 infection after inoculation with the infected rabbit T cell line RH/K34 (see ATCC CRL-2560). [50754]

Approximately 20 months after inoculation, the rabbit developed a generalized exfoliative papillated dermatopathy. Cutaneous lesions were biopsied. [50754]

The cell line harbors productively infected, monoclonally integrated, full length HTLV-1.

The HTLV-1 sequences in the skin likely represent the presence of infected T cells in epidermotropic lymphoid infiltrates. [50754]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with an additional 2 mM L-glutamine, 80%; fetal bovine serum, 20%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 X 10(5) viable cells/ml.

Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml.

Medium Renewal: RHCT-138細(xì)胞Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50754: Simpson RM, et al. Cutaneous manifestations of human T cell leukemia virus type I infection in an experimental model. J. Infect. Dis. 173: 722-726, 1996. PubMed: 8627039

53506: Kindt TJ, et al. Passage of human T-cell leukemia virus type-1 during progression to cutaneous T-cell lymphoma results in myelopathic disease in an HTLV-1 infection model. Microbes Infect. 2: 1139-1146, 2000. PubMed: 11008104