34細(xì)胞
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： IgG2b , IgG2b
ATCC Number： CRL-1889?
數(shù)量： 大量
Designations: 34
Depositors: B Chesebro
Isotype: IgG2b , IgG2b
34細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against p15 group antigen (GAG) protein of murine leukemia viruses (MuLV)
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Comments: Spleen cells from a mouse recovering from erythroleukemia induced by a B-tropic Friend murine leukemia virus were fused with NS-1 myeloma cells.
34細(xì)胞The antibody reacts with the p15 protein from several MuLV including Friend, Moloney, Rauscher, amphotropic and Spleen Focus Forming (SFFV) viruses.
The antibody reacts with infected cells after acetone fixation and immunoprecipitates p15.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
0.05 mM 2-mercaptoethanol
Temperature: 37.0°C
Subculturing: Protocol: 34細(xì)胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1778: Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798
1779: Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. 34細(xì)胞PubMed: 6305011
