El Don細(xì)胞

ATCC Number： CRL-1149?

相關(guān)**： 其他**

年限： 31 years

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來源： 皮膚

是否是腫瘤細(xì)胞： 0

物種來源： 人

El Don細(xì)胞數(shù)量： 大量

細(xì)胞形態(tài)： 成纖維樣

Designations: El Don

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: El Don細(xì)胞Ehlers-Danlos syndrome, type VII (arthrocalasis type)

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Age: 31 years

Gender: female

Comments: The tissue donor suffered from type VII Ehlers-Danlos syndrome (arthrocalasis type), and was patient No. 2 in Lichtenstein, JR, et al. Science 182:298, 1973.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. El Don細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.

References: 22242: Lichtenstein JR, et al. El Don細(xì)胞Defect in conversion of procollagen to collagen in a form of Ehlers-Danlos syndrome. Science 182: 298-300, 1973. PubMed: 4742738