RH/K34細胞

ATCC Number： CRL-2561?

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 其他

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

器官來源： 外周血

運輸方式： 凍存運輸

數(shù)量： 大量

Designations: RH/K34

Depositors: RM Simpson

RH/K34細胞Biosafety Level: 2 [Cells infected with HTLV-I virus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates and some adherent cells

Organism: Oryctolagus cuniculus deposited as rabbit

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: lymphocyteinfected with HTLV-I

Cellular Products: HTLV-I (human T cell leukemia virus)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RH/K34細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: 1. This cell line was deposited at the ATCC by Dr. R. Mark Simpson and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of these cells, or their products must first be negotiated with the National Institute of Allergy and Infectious Diseases (NIAID) Office of Technology Development (OTD), Building 31, Room 3B-62 31 Center Drive, MSC 2137 Bethesda, MD 20892-2137 TEL: 301-496-2644 FAX: 301-402-7123

Isolation: Isolation date: 1991

Receptors: T cell antigen receptor gamma (TCR), not expressed; interleukin-2 receptor (IL-2R) + [50673]

Virus Susceptibility: Human immunodeficiency virus 1 (HIV-1) (weakly)

Antigen Expression: CD5 +/-; CD4 -; CD8 -; MHC class II + [50673]

Gender: unknown

Comments: The RH/K34 gamma/delta T cell line was derived from rabbit peripheral blood mononuclear cells (PBMC) by coculture with the rabbit RHT-16 cell line. RH/K34細胞[50673]

RHT-16 contains integrated HTLV-1 leukemia virus-I (HTLV-1) provirus and sheds intact virus particles.

The RH/K30 (ATCC CRL-2560) and RH/K34 (ATCC CRL-2561) cell lines differ in their potential to cause disease in rabbits.

Injection of the RH/K34 cell line causes human ***** T-cell leukemia/lymphoma-like (ATLL) disease that is fatal within 10 days.

However, all rabbits injected with the same or higher doses of RH/K30 survived with a low-grade leukemia that showed evidence of acute rejection. [50677]

The cell line may be used as an infectious human disease model; it can be used to infect rabbits with HTLV-1.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml. Do not allow the cell concentration to exceed 2 X 10(6) cells/ml.

Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml.

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50673:RH/K34細胞 Sawasdikosol S, et al. Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I. J. Exp. Med. 178: 1337-1345, 1993. PubMed: 8376938

50677: Zhao TM, et al. Variation in HTLV-I sequences from rabbit cell lines with diverse in vivo effects. Virology 195: 271-274, 1993. PubMed: 8317104

50751: Mahana W, et al. Genes in the pX region of human T cell leukemia virus I influence Vav phosphorylation in T cells. Proc. Natl. Acad. Sci. USA 95: 1782-1787, 1998. PubMed: 9465094

50752: Simpson RM, et al. Experimental acute ***** T cell leukemia-lymphoma is associated with thymic atrophy in human T cell leukemia virus type I infection. Lab. Invest. 74: 696-710, 1996. PubMed: 8600320

50753: Chouchane L, et al. Heat-shock proteins expressed on the surface of human T cell leukemia virus type I-infected cell lines induce autoantibodies in rabbits. J. Infect. Dis. 169: 253-259, 1994. PubMed: 7508967