EL 1細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

器官來(lái)源： 脾

細(xì)胞形態(tài)： 單核細(xì)胞/巨噬細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

ATCC Number： CRL-9854?

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

Designations: EL 1

EL 1細(xì)胞Depositors: E.I. du Pont de Nemours & Co.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: monocyte/macrophage

Source: Organ: spleen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: EL 1細(xì)胞The EL 1 cell line was established from cryopreserved human spleen cells.

The cells tend to grow as single cells but may form small nonadherent clusters.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; calf serum, 10%

Temperature: 37.0°C

EL 1細(xì)胞Subculturing: Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml)

Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

References: 26300: Collins GW, Largen MT. EL 1細(xì)胞Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995