32B11細(xì)胞
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
**類型： IgM; kappa light chain
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
ATCC Number： CRL-2559?
運(yùn)輸方式： 凍存運(yùn)輸
Designations: 32B11
32B11細(xì)胞Depositors: SF Wright
Isotype: IgM; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against glomalin (a glycoprotein in soil)
Permits/Forms: 32B11細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with freshly collected Glomus intraradices FL208 spores. [50654]
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody reacts with glomalin on the surface of hyphae and spores and also on glomalin sloughed from hyphae.
The antibody is slightly cross-reactive with Leptospharia korrea.
Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. It is found worldwide and it may the primary glue that holds soil together.
The antibody is used for studies on soil structure; it is useful in ELISA assays to detect glomalin on the surface of hyphae and glomalin in root cortical cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
32B11細(xì)胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Attached cells may be subcultured by scraping. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: culture medium 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 50654: Wright SF, et al. 32B11細(xì)胞Time-course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during active colonization of roots. Plant Soil 181: 193-203, 1996.
